C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/20 (2006.01) A21D 8/04 (2006.01) C11D 3/386 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/12 (2006.01) C12N 15/09 (2006.01) A23L 1/105 (2006.01)
Patent
CA 2353379
The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
La présente invention concerne un procédé de modification de la spécificité du substrat d'une enzyme lipolytique par altération de la séquence amino-acide dans une zone définie de l'enzyme lipolytique, de manière à augmenter le taux d'une activité souhaitée ou de baisser le taux d'une activité souhaitée. Ainsi, l'invention permet de mettre au point des variants d'enzyme lipolytique comprenant une séquence modifiée amino-acide avec une spécificité de substrat pouvant s'adapter à des utilisations spécifiques.
Bojsen Kirsten
Borch Kim
Budolfsen Gitte
Fuglsang Klaus Crone
Glad Sanne Schroder
Dimock Stratton Llp
Novozymes A/s
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