Lipolytic enzyme variants and method for their production

Details Lipolytic enzyme variants and method for their production Lipolytic enzyme variants and method for their production

C - Chemistry, Metallurgy

12

N

C12N 9/20 (2006.01) A21D 8/04 (2006.01) C12N 9/16 (2006.01) C12N 9/18 (2006.01)

Patent

CA 2467415

The inventors have developed a method using protein engineering to produce lipolytic enzymes having a relatively high activity for one ester bond in an amphiphilic substrate with two lipophilic groups) and a relatively low activity for the ester bond in an amphiphilic substrate with one lipophilic group, e.g. a relatively high phospholipase activity and a relatively low lysophospholipase activity.

L'invention concerne un procédé dans lequel on utilise l'ingéniérie des protéines pour produire des enzymes lipolytiques avec une activité relativement élevée pour une liaison ester dans un substrat amphiphile avec deux groupes lipophiles, et une activité relativement faible pour la liaison ester dans un substrat amphiphile avec un groupe lipophile, par exemple une activité phospholipase relativement élevée et une activité lysophospholipase relativement faible.

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