C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/16 (2006.01) C07K 7/08 (2006.01) C07K 7/56 (2006.01) C12N 9/80 (2006.01) C12P 21/04 (2006.01)
Patent
CA 2043762
Echinocandin B deacylase, a cell-associated enzyme from Actinoplanes utahensis, is purified to near homogeneity in a process comprising hydrophobic interaction chromatography, cation-exchange chromatography and gel filtrations. The enzyme is a heterodimer containing 18-KD and 63-KD subunits and is a simple enzyme unaffected in activity by co-factors, metal chelators, or sulfhydryl reagents. The enzyme catalyzes the deacylation of the lipid aryl portion of lipid cyclicpeptide metabolites such as ECB and aculeacin.
Kreuzman Adam J.
Yeh Wu-Kuang
Eli Lilly And Company
Gowling Lafleur Henderson Llp
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