Lipoprotein analysis by differential charged-particle mobility

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 1/14 (2006.01) C07K 1/22 (2006.01) G01N 1/28 (2006.01) G01N 15/02 (2006.01) G01N 27/62 (2006.01) C07K 14/775 (2006.01)

Patent

CA 2690305

The invention provides apparatus and methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged-particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged-particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.

L'invention concerne un appareil et des procédés de préparation de lipoprotéines à partir d'un échantillon biologique, notamment HDL, LDL, Lp(a), IDL, et VLDL, à des fins de diagnostic en utilisant des procédés d'analyse de la mobilité différentielle de particules chargées. Elle concerne également des procédés d'analyse de la distribution de tailles des lipoprotéines par mobilité différentielle de particules chargées, les lipoprotéines étant préparées par des procédés de l'invention. Elle concerne aussi des procédés pour évaluer les risques en matière de santé liés aux lipides, une condition cardiovasculaire, le risque de maladie cardiovasculaire et la capacité de réponse à une intervention thérapeutique, ces procédés employant la distribution de tailles des lipoprotéines déterminée par des procédés de l'invention.

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