Lipoprotein assay

G - Physics – 01 – N

Patent

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Details

G01N 33/92 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2655010

The present invention concerns a method of determining the concentration of total lipoprotein in a sample. The method involves the steps of: (i) adding to an aliquot of the sample a lipophilic dye that binds to lipoproteins in the sample and which when so bound fluoresces under appropriate excitation; and (ii)determining the total lipoprotein concentration in the sample using fluorescence analysis. A method of analysing the lipoprotein content of a sample solution using a dye that discriminates between different types of lipoprotein is also disclosed.

La présente invention concerne un procédé de détermination de la concentration en lipoprotéines totales dans un échantillon. Le procédé comprend les étapes suivantes : (i) ajouter à une partie aliquote de l'échantillon un colorant lipophile qui se lie aux lipoprotéines de l'échantillon et qui, quand il est ainsi lié, devient fluorescent sous une excitation appropriée; et (ii) déterminer la concentration en lipoprotéines totales dans l'échantillon par l'analyse de la fluorescence. L'invention concerne également un procédé d'analyse de la teneur en lipoprotéines d'une solution échantillon en utilisant un colorant qui différencie les différents types de lipoprotéines.

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