Liposome compatible surfactants

Details Liposome compatible surfactants Liposome compatible surfactants

G - Physics

01

N

167/45

G01N 33/53 (2006.01) G01N 33/532 (2006.01) G01N 33/566 (2006.01) G01N 33/58 (2006.01) G01N 35/00 (2006.01)

Patent

CA 1258627

ABSTRACT Disclosed is a specific binding assay composition and method for determining a ligand in a sample. The composition comprises (a) a binding partner for the ligand; (b) a detection system which has at least two components; (c) a selectively accessible vesicle having a surface-incorporated ligand or ligand analog and a first component of the detection system therein; (d) a substance which modifies vesicle accessibillty in response to binding of surface-incorporated ligand or ligand analog and the binding partner; (e) at least one additional component of the detection system which is reactive with the first component to produce a detectable response; and (f) at least one surfactant which does not modify vesicle accessibil- ity. The composition and method are suitable for use with automated, including continuous flow-type, analyzers.

485358

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