Liposomes containing oligonucleotides

A - Human Necessities – 61 – K

Patent

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Details

A61K 48/00 (2006.01) A61K 9/127 (2006.01) A61K 31/70 (2006.01) C07H 21/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 15/11 (2006.01) C12Q 1/68 (2006.01) G01N 33/92 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2288613

It is possible to radiosensitize tumor cells by administration of compositions containing the Human antisense <u>c-raf-1</u> oligodeoxyribonucleotide (ODN/oligo) sequence: 5'-GTGCTCCATTGATGC-3' (seq. #1) wherein only the end bases are phosphorylated is a preferred embodiment. Antisense sequences of up to 40 bases which contain this sequence may be used in accordance with the teachings of this disclosure. Compositions comprising a cationic liposome of dimethyldioctadecyl ammonium bromide, phosphatidylcholine and cholesterol may be used as a carrier system. The liposomes provide a new carrier system that is particularly useful for administration of sequences for therapy.

Il est possible de radiosensibiliser des cellules tumorales en administrant des compositions renfermant la séquence oligodéoxyribonucléotide (ODN/oligo) anti-sens humaine <u>c-raf-1</u>: 5'-GTGCTCCATTGATGC-3' (séquence No.1), dans laquelle, selon un mode de réalisation préféré, seules les bases terminales sont phosphorylées. Des séquences anti-sens qui comprennent jusqu'à 40 bases renfermant cette séquence peuvent être utilisées conformément aux enseignements de cette invention. Des compositions renfermant un liposome cationique de bromure d'ammonium diméthyldioctadécyle, de phosphatidylcholine, et de cholestérol peuvent être utilisées en tant que système porteur. Les liposomes fournissent un nouveau système porteur particulièrement utile à l'administration thérapeutique de séquences.

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