Liposomes prepared from the extractable lipids from a...

A - Human Necessities – 61 – K

Patent

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Details

A61K 39/39 (2006.01) A61K 9/127 (2006.01) A61P 35/00 (2006.01) A61P 37/04 (2006.01)

Patent

CA 2455597

The invention relates to a liposome comprising a chloroform soluble and extractable total polar lipid of Mycobacterium spp, particularly a chloroform soluble extractable total polar lipid of Mycobacterium spp BCG. The chloroform soluble and extractable polar lipid may comprise at least one of phosphatidylinositol (PI), phosphatidylinositol mannoside (PIM1), phosphatidylinositol dimannoside (PIM2), mono and dipalmitoylated forms of PIM1 and PIM2, phospholipid of 899 m/z, phosphatidylethanolamine and cardiolipid. The liposome may be prepared by drying chloroform soluble and extractable lipid and then hydrating said dried lipid at a temperature of 65 to 75 ~C in water or phosphate buffered saline (PBS). The liposome may be used, for example, to activate dendritic cells to secrete cytokines and modulate an immune response in a mammal, or to direct an immune response to confer protection against a pathogen or a cancer.

L'invention concerne un liposome comprenant un lipide polaire total soluble dans le chloroforme et extractible au chloroforme de Mycobacterium spp, en particulier un lipide polaire total soluble dans le chloroforme et extractible au chloroforme de Mycobacterium bovis BCG. Le lipide polaire soluble dans le chloroforme et extractible au chloroforme peut comprendre au moins un phosphatidylinositol (PI), un phosphatidylinositol mannoside (PIM¿1?), un phosphatidylinositol dimannoside (PIM¿2?), des formes mono et dipalmitoylées de PIM¿1? et PIM¿2?, un phospholipide de 899 m/z, un phosphatidylethanolamine et un cardiolipide. Ce liposome peut être préparé selon une technique consistant à sécher le lipide soluble dans le chloroforme et extractible au chloroforme puis à l'hydrater à une température comprise entre 65 et 75 ·C dans l'eau ou dans une solution saline tamponnée au phosphate (PBS). Le liposome peut être utilisé, par exemple, pour activer les cellules dendritiques afin que celles-ci sécrètent des cytokines et modulent la réponse immune chez un mammifère, ou pour diriger une réponse immune afin d'obtenir une protection contre un agent pathogène ou un cancer.

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