Liquid chromatography using microporous hollow fibers

Details Liquid chromatography using microporous hollow fibers Liquid chromatography using microporous hollow fibers

B - Operations, Transporting

01

D

182/2, 252/23

B01D 15/08 (2006.01) B01D 69/08 (2006.01) B01J 20/22 (2006.01) G01N 30/60 (2006.01)

Patent

CA 2002370

ABSTRACT Liquid chromatography separations of solutes are achieved using porous hollow fibers. The pores of the hollow fibers immobilize a solute-absorbing phase (preferably organic) which has a greater absorbance affinity towards at least one solute in a mixture of solutes. By passing the solute mixture through the central lumen of the hollow fibers, chromatographic separations are realized due to the greater retention time of that solute with which the immobilized phase has greater absorbance affinity. The immobilized phase may be a liquid organic which may contain a surfactant so as to form reversed micelles or it may be in the form of a polymeric gel. Separations of biological species (e.g., proteins) may thus be accomplished by means of the present invention.

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