Liquid/gas phase exposure reactor for cell cultivation

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 3/06 (2006.01)

Patent

CA 2568646

The invention relates to a method and a device for the raising of cells and cell cultivation in high density, whereby the cells for cultivation are located in hollow fibre membranes and are alternately supported in a liquid nutrient and a gas phase thereabove. The device is a liquid/gas phase exposure bioreactor with a supply chamber, in which hollow fibre membranes with an inner diameter of no more than 5 mm are located and the inner volumes of which form culture chambers. After introduction of the cells into the culture chambers approximately half of the supply chamber is filled with nutrient medium and the other half with a gas mixture. After switching on the medium and gas perfusion, a cyclic exposure of the hollow fibre membranes and the cells therein to the gas or the liquid phase begins.

L'invention concerne un procédé et un dispositif pour la culture et la propagation de cellules dans des concentrations élevées, les cellules à cultiver se trouvant dans des membranes à fibres creuses et étant introduites en alternance dans un milieu de culture liquide et dans une phase gazeuse se trouvant au-dessus de ce dernier. Ledit dispositif est un bioréacteur d'exposition à une phase liquide-gazeuse comportant une chambre d'alimentation, dans laquelle se trouvent des membranes à fibres creuses qui présentent un diamètre intérieur inférieur ou égal à 5 mm et dont les volumes intérieurs forment des chambres de culture. Après introduction des cellules dans les chambres de culture, environ la moitié de la chambre d'alimentation est remplie de milieu de culture, l'autre moitié étant remplie d'un mélange gazeux. Après le début de la perfusion du milieu et du gaz, les membranes à fibres creuses et les cellules se trouvant à l'intérieur de ces dernières commencent à être exposées de manière cyclique à la phase gazeuse et liquide.

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