Liquid reagent set for l-lactate determination

Details Liquid reagent set for l-lactate determination Liquid reagent set for l-lactate determination

C - Chemistry, Metallurgy

12

Q

C12Q 1/26 (2006.01) C12Q 1/28 (2006.01) G06F 11/36 (2006.01)

Patent

CA 2334276

The invention provides sets of liquid reagents that afford cal-ibration stability in an enzyme based spectrophotometric assay for the measurement of lactate in patient samples. The reagent sets include a lactate oxidase, a peroxidase, a hydrogen donor, an agent that substantially prevents ascorbic acid interference, and agent that substantially prevents bilirubin interference, a coupling agent, a buffer and, optionally, a preservative. The invention fur-ther provides methods for using the liquid reagent sets.

L'invention concerne des ensembles de réactifs liquides présentant un facteur d'étalonnage stable dans un dosage spectrophotométrique à base d'enzymes, ces ensembles permettant d'effectuer une détermination quantitative du lactate dans des échantillons prélevés sur des patients. Ces ensembles de réactifs comprennent une lactate oxydase, une peroxydase, un donneur d'hydrogène, un agent destiné à prévenir sensiblement toute interférence de l'acide ascorbique, un agent destiné à prévenir sensiblement toute interférence de la bilirubine, un agent de couplage, un tampon, et éventuellement un agent de conservation. Cette invention concerne également des méthodes d'utilisation de ces ensembles de réactifs liquides.

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