Liquid single reagent for assays

Details Liquid single reagent for assays Liquid single reagent for assays

G - Physics

01

N

167/37

G01N 33/546 (2006.01) G01N 33/543 (2006.01) G01N 33/549 (2006.01) G01N 33/555 (2006.01)

Patent

CA 1308020

ABSTRACT OF THE DISCLOSURE Assay methods and compositions are provided for determining an analyte in a sample suspected of containing the analyte. The composition comprises in a novel single liquid reagent at least one specific binding pair (sbp) member and its complementary member wherein at least one sbp member is reversibly confined in a material that temporarily renders the confined sbp member incapable of binding with its complementary sbp member. At least one of the sbp members is bound to a member of a signal producing system capable of producing a detectable signal in relation to the amount of analyte in the sample. The confinement is reversed, any remaining members of the signal producing system are added, and the signal produced in relation to the amount of analyte is measured.

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