G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/53 (2006.01) C07K 16/00 (2006.01) C12N 15/00 (2006.01) C12P 21/04 (2006.01) G01N 33/574 (2006.01)
Patent
CA 2518569
The current invention provides a method for directly converting histopathologically processed biological samples, tissues, and cells into a multi-use biomolecule lysate. This method allows for simultaneous extraction, isolation, solublization, and storage of all biomolecules contained within the histopathologically processed biological sample, thereby forming a representative library of said sample. This multi-use biomolecule lysate is dilutable, soluble, capable of being fractionated, and used in any number of subsequent experiments.
L'invention concerne un procédé pour convertir directement des échantillons, des tissus et des cellules biologiques issus d'un procédé histopathologique en un lysat multi-usager de biomolécules. Ce procédé permet l'extraction, l'isolation, la solubilisation et le stockage simultanés de toutes les biomolecules dans un échantillon biologique issu d'un procédé histopathologique, en réalisant ainsi une collecte représentative de cet échantillon. On peut diluer, dissoudre, fractionner et utiliser ce lysat multi-usager de biomolécules pour un nombre illimité d'expériences subséquentes.
Darfler Marlene M.
Krizman David B.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Expression Pathology Inc.
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