Lithium salts as red blood cells lysing hemoglobin...

Details Lithium salts as red blood cells lysing hemoglobin... Lithium salts as red blood cells lysing hemoglobin...

G - Physics

01

N

167/37

G01N 33/53 (2006.01) G01N 33/50 (2006.01) G01N 33/72 (2006.01)

Patent

CA 2019426

Use of a lithium salt, particularly lithium thiocyanate, as an agent for lysing red blood cells and denaturing hemoglobin in the performance of an immunoassay to detect the relative amount of a hemoglobin derivative in a blood sample. The method is particularly useful in the determination of hemoglobin Alc. The method provides a rapid means for releasing hemoglobin from red blood cells by lysis and for exposing the epitope that is characteristic of the Alc form. Low concentrations of the lithium salt provide rapid release and denaturation of hemoglobin without the need for dilution prior to the performance of the immunoassay.

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