Long wavelength thiol-reactive fluorophores

Details Long wavelength thiol-reactive fluorophores Long wavelength thiol-reactive fluorophores

C - Chemistry, Metallurgy

09

B

C09B 57/00 (2006.01) C09B 23/10 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2567387

Reactive fluorescent dyes compositions and methods of using same are disclosed. Squaraine nucleus, Nile red nucleus, benzodioxazole nucleus, coumarin nucleus or aza coumarin nucleus dyes are disclosed dyes are disclosed having thiol-reactive groups. Squaraine nucleus, Nile Red nucleus, benzodioxazole nucleus, coumarin nucleus or aza coumarin nucleus dyes are disclosed that exhibit a fluorescence emission of at least about 575 nm. Biosensors are disclosed having a binding protein and a squaraine nucleus, Nile Red nucleus, benzodiaxazole nucleus, coumarin nucleus or aza coumarin nucleus.

L'invention concerne des compositions fluorescentes réactives et des procédés d'utilisation correspondants. Elle porte sur des colorants à base d'un noyau de squaraine, d'un noyau de rouge de Nile, d'un noyau de benzodioxazole, d'un noyau de coumarine ou d'aza coumarine, ces colorants possédant des groupes réactifs avec thiol. Les colorants à base d'un noyau de squaraine, d'un noyau de rouge de Nile, d'un noyau de benzodioxazole, d'un noyau de coumarine ou d'aza coumarine manifestent une émission de fluorescence d'au moins environ 575 nm. L'invention concerne aussi des biocapteurs qui possèdent une protéine de liaison et un noyau de squaraine, un noyau de rouge de Nile, un noyau de benzodioxazole, ou un noyau de coumarine ou d'aza coumarine.

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