Low cost manufacture of oligosaccharides

Details Low cost manufacture of oligosaccharides Low cost manufacture of oligosaccharides

C - Chemistry, Metallurgy

12

N

C12N 9/10 (2006.01) C07H 21/04 (2006.01) C12N 1/20 (2006.01) C12N 15/00 (2006.01) C12P 19/18 (2006.01)

Patent

CA 2351022

This invention provides recombinant cells, reaction mixtures, and methods that are useful for the enzymatic synthesis of product saccharides. The recombinant cells contain a heterologous gene that encodes a glycosyltransferase which catalyzes at least one step of the enzymatic synthesis, as well a system for generating a nucleotide sugar that can serve as a substrate for the glycosyltransferase. The recombinant cells, reaction mixtures, and methods are useful for efficiently synthesizing a large variety of saccharides, including polysaccharides, oligosaccharides, glycoproteins and glycolipids, using relatively low-cost starting materials.

L'invention concerne des cellules de recombinaison, des mélanges de réaction, et des techniques utilisées pour la synthèse enzymatique des saccharides de production. Les cellules de recombinaison contiennent un gène hétérologue codant pour une glycosyltransférase qui catalyse au moins une étape de la synthèse enzymatique, ainsi qu'un système permettant de produire un sucre de nucléotide pouvant servir de substrat à la glycosyltransférase. Ces cellules de recombinaison, ces mélanges de réaction, et ces techniques sont utilisés pour réaliser efficacement la synthèse d'une grande variété de saccharides, notamment des polysaccharides, des oligosaccharides, des glycoprotéines et des glycolipides, au moyen de produits de départ relativement bon marché.

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