Low electroendosmosis agarose

Details Low electroendosmosis agarose Low electroendosmosis agarose

B - Operations, Transporting

01

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B01J 20/24 (2006.01) B01D 57/02 (2006.01) C07K 1/24 (2006.01) C07K 1/26 (2006.01) C08B 37/00 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2060314

ABSTRACT: Purified agarose suitable for rapid electrophoresis, characterized by a sulfate content of less than 0.2 wt % but greater than zero, a pyruvate content of 0-0.1 wt %, and a nitrogen content of 0-0.02 wt %. Gels prepared from the agarose exhibit a gel strength at 1.0 wt % concentration of at least 1200 g/cm2, substantial absence of DNA binding in 0.07 M or less tris acetate buffer, and an electroendosmosis (EEO) at 1.0 wt % concentration of 0.05 or less. Agaroses are purified to provide the low EEO material by dissolving agarose or alkali-modified agar in an aqueous medium buffered at a pH of 6.0 to 8.0 and containing no more than 2.0 mM salt as chloride, and precipitating the agarose by contact with a lower alkanol.

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