Low light color imaging system with cooled integrating camera

H - Electricity – 04 – N

Patent

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H04N 5/335 (2006.01) G01N 21/64 (2006.01) H04N 5/225 (2006.01) G01N 15/14 (2006.01)

Patent

CA 2139754

A low light level color imaging system acquires po- lychromatic images of body cells at low light intensities when markers attached to the cells are illuminated. Particu- larly in PAP smear sceening, specimens may be marked with a variety of fluorescent stains which have different chromatic signatures but which only weekly emit light from very low structural le- vels of the cells. The inven- tion detects the color intensi- ty of each stain in a specimen image, using an integrating color camera to acquire the image. The signal-to-noise ra- tio of the integrating color camera is enhanced by elec- tronic cooling (50, etc.) of its imaging element (28). A single scanned image of a specimen acquired by the camera includes polychromatic information which identifies all of the fluorescent stains which mark the specimen. A multi-chan- nel color image analyzer is configured to recognize each of the fluorescent colors produced from the fluorescent dyes and to pro- vide a pixellated representation of the specimen image which includes all of the fluorescent colors at their image locations.

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