Low temperature albumin fractionation using sodium caprylate...

Details Low temperature albumin fractionation using sodium caprylate... Low temperature albumin fractionation using sodium caprylate...

C - Chemistry, Metallurgy

07

K

C07K 14/765 (2006.01) C07K 1/30 (2006.01) C07K 1/34 (2006.01) A61K 38/38 (2006.01)

Patent

CA 2155630

Highly stable plasma-derived therapeutic albumin solutions, having a turbidity level of 5 NTU or less can be made by adding sodium caprylate to Cohn fraction II+III or IV-1 effluent at relatively low temperatures. The sodium caprylate acts as a partitioning agent to separate albumin from unwanted proteins. In preferred embodiments, the albumin source solution temperature is elevated, increased in pH and reacted for approximately six hours under conditions sufficient to disrupt the initial solution colloid, and partition albumin-containing supernatant from a colloidal disperse phase, which retains unwanted globulins and manufacturing debris. Since it tends to be a scavenger molecule, albumin is selectively stabilized by diafiltration against a buffer containing sodium caprylate, thereby assuring a high albumin monomer content and low turbidity level. The amount of sodium caprylate required for selective stabilization is determined by the amount of available binding sites on the albumin molecule.

Affiliated with

Also associated with

Rating

Low temperature albumin fractionation using sodium caprylate... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Low temperature albumin fractionation using sodium caprylate..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Low temperature albumin fractionation using sodium caprylate... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1935850