Low-temperature inducible expression vector

Details Low-temperature inducible expression vector Low-temperature inducible expression vector

C - Chemistry, Metallurgy

12

N

C12N 15/70 (2006.01) C07K 14/245 (2006.01) C12N 9/24 (2006.01) C12N 15/00 (2006.01) C12N 15/63 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2309600

A vector which is characterized in containing each of the following elements: (1) a promoter which shows its action in the host to be used; (2) regulatory region for regulating the action of the promoter of (1); and (3) a region which codes for the 5' -untranslated region derived from cold-shock protein gene mRNA or a region which codes for the region where substitution, deletion, insertion or addition of at least one base is applied to the said untranslated region.

Vecteur qui possède les éléments suivants: (1) un promoteur qui fonctionne dans un hôte à utiliser, (2) une région régulatrice destinée à réguler la fonction du promoteur (1), et (3) une région codant pour une région 5' non traduite dérivée d'ARNm du gène de protéine de choc thermique froid ou une région codant pour une région formée en soumettant la région non traduite à la substitution, la suppression ou l'ajout d'au moins une base.

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