Luciferase reporter bioassay of parathyroid hormone compounds

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C12Q 1/66 (2006.01) G01N 33/566 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2342926

This invention is directed to a bioassay for determining the functionality of parathyroid hormone compounds. More particularly, this invention is directed to a bioassay wherein the compound to be tested is added to a culture of parathyroid hormone receptor expressing cells bearing a reporter gene under the transcriptional control of multiple c- AMP responsive elements.

L'invention concerne un essai biologique visant à déterminer la fonctionnalité de composés à base d'hormone parathyroïde. Plus précisément, l'invention concerne un essai biologique dans lequel le composé à tester est ajouté à une culture de cellules exprimant le récepteur de l'hormone parathyroïde qui comportent un gène récepteur, sous le contrôle transcriptionnel de plusieurs éléments réceptifs à l'AMP cyclique.

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