Luminescent markers

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/62 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C07K 19/00 (2006.01) C12N 5/10 (2006.01) C12N 15/12 (2006.01) G01N 21/00 (2006.01) G01N 33/53 (2006.01) G01N 33/542 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2482126

The invention relates to luminescent proteins, nucleic acids encoding same, compositions and combinations comprising the proteins, and methods using the proteins, nucleic acids, compositions and combinations. In particular, a luminescent protein is provided comprising oncomodulin in which a salt bridge has been introduced to provide greater stability. The protein may be used as a luminescent marker in, for example, luminescent items, immunoassays, and fluorescent energy transfer assays.

La présente invention a trait à des protéines luminescentes, des acides nucléiques codant pour lesdites protéines, des compositions et des combinaisons comprenant les protéines, et des procédés mettant en oeuvre les protéines, acides nucléiques, compositions et combinaisons. En particulier, l'invention a trait à une protéine luminescente comprenant une oncomoduline dans laquelle un pont de sel a été introduit pour lui conférer une stabilité accrue. La protéine peut être utilisée en tant que marqueur luminescent, par exemple, dans des articles, des immuno-essais fluorescents et dans des dosages de transfert d'énergie fluorescente.

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