Lyase enzymes, nucleic acids encoding them and methods for...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/60 (2006.01) A62D 3/02 (2007.01) G06F 19/22 (2011.01) A01H 5/00 (2006.01) A01K 67/027 (2006.01) A01N 63/02 (2006.01) A01P 1/00 (2006.01) A21D 8/04 (2006.01) A23C 9/12 (2006.01) A23K 1/165 (2006.01) A23L 1/305 (2006.01) A23L 2/66 (2006.01) A61K 38/51 (2006.01) A61L 2/16 (2006.01) C02F 3/00 (2006.01) C07H 21/00 (2006.01) C07K 16/40 (2006.01) C07K 17/00 (2006.01) C07K 19/00 (2006.01) C11D 3/386 (2006.01) C11D 7/42 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 5/16 (2006.01) C12N 9/88 (2006.01)

Patent

CA 2653967

This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.

L'invention concerne des polypeptides ayant une activité lyase, des polynucléotides codant pour ces polypeptides, et des procédés de préparation et d'utilisation de ces polynucléotides et ces polypeptides. Dans un aspect, l'invention concerne des polypeptides ayant une activité lyase d'ammoniaque, par exemple une activité lyase d'ammoniaque de phénylalanine, lyase d'ammoniaque de tyrosine et/ou lyase d'ammoniaque d'histidine, comprenant une activité thermostable et thermotolérante, et des polynucléotides codant ces enzymes, et la préparation et l'utilisation de ces polynucléotides et polypeptides. Les polypeptides de l'invention peuvent être utilisés dans une variété de contextes pharmaceutiques, agricoles et industriels.

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