Lyase enzymes, nucleic acids encoding them and methods for...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/88 (2006.01)

Patent

CA 2614769

This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.

L'invention concerne des polypeptides à activité lyase, des polynucléotides codant pour ces polypeptides, et des méthode de fabrication et d'utilisation desdits polynucléotides et polypeptides. Dans un aspect, l'invention concerne des polypeptides présentant une activité ammonia lyase, par exemple une activité phénylalanine ammonia lyase, tyrosine ammonia lyase et/ou histidine ammonia lyase, y compris une activité thermostable et thermotolérante, et des polynucléotides codant pour ces enzymes, ainsi que la fabrication et l'utilisation de ces polynucléotides et polypeptides. Les polypeptides de l'invention peuvent être utilisés dans divers contextes pharmaceutiques, agricoles et industriels.

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