Lymphatic endothelial cells materials and methods

Details Lymphatic endothelial cells materials and methods Lymphatic endothelial cells materials and methods

C - Chemistry, Metallurgy

12

N

C12N 5/078 (2010.01) A61K 35/14 (2006.01) A61K 39/395 (2006.01) A61K 48/00 (2006.01) C12Q 1/02 (2006.01) G01N 33/567 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2453161

The present invention is directed to methods and compositions for isolating lymphatic endothelial cells from a mixed population of cells. More particularly, the inventors have found that certain antibodies that recognize the extracellular domain of VEGFR-3 can be used to specifically isolated lymphatic endothelial cells substantially free of other contaminating non- lymphatic endothelial cells. Methods and compositions for producing such cells and using such cells are described.

L'invention concerne des procédés et des compositions d'isolation de cellules endothéliales lymphatiques issues d'une population mélangée de cellules. Plus particulièrement, les inventeurs ont constaté que certains anticorps qui reconnaissent le domaine extracellulaire de VEGRF-3 peuvent être utilisés sur des cellules endothéliales lymphatiques spécialement isolées sensiblement dépourvues d'autres cellules endothéliales non-lymphatiques contaminantes. L'invention porte aussi sur des procédés et des compositions de production desdites cellules et d'utilisation de ces dernières.

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