Lymphoid tissue-specific cell production from hematopoietic...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/078 (2010.01) C12N 5/0783 (2010.01) A61K 35/12 (2006.01) A61L 27/38 (2006.01) C12Q 1/02 (2006.01) C12Q 1/24 (2006.01)

Patent

CA 2351889

The invention relates to a method for lymphoid tissue-specific cell production from hematopoietic progenitor cells in unique, three-dimensional culture devices, in the presence of lymphoreticular stromal cells and in the absence of exogenously added growth factors. The resulting differentiated progeny. The lymphoid tissue-specific cells may be isolated at any sequential stage of differentiation and further expanded. The lymphoid tissue-specific cells also may be genetically altered at any stage of the process.

L'invention porte sur un procédé de production de cellules lymphoïdes spécifiques d'un tissu à partir de cellules souches hématopoïétiques se trouvant dans des dispositifs tridimensionnels uniques, en présence de cellules lymphoréticulaires du stroma et en l'absence de facteurs de croissance exogènes ajoutés. On obtient ainsi une descendance différenciée. Les cellules lymphoïdes spécifiques d'un tissu peuvent être isolées à n'importe quelle étape séquentielle de la différenciation et ensuite développées. Les cellules lymphoïdes spécifiques d'un tissu peuvent être également modifiées génétiquement à toute étape du processus.

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