Lymphotoxin dna, lymphotoxin expression vector, lymphotoxin...

C - Chemistry – Metallurgy – 12 – N

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195/1.2, 195/1.2

C12N 15/19 (2006.01) C07K 14/525 (2006.01) C12N 5/10 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1302921

Abstract of the Disclosure A chromosomal DNA sequence which codes for human lymphotoxin, a lymphotoxin expression vector which contains a DNA sequence wherein a chromosomal DNA sequence coding for human lymphotoxin and promoter region which functions in animal cell are linked together, lymphotoxin resistant cell line, transformed animal cell culture which is formed by transforming cultured animal cell with a lymphotoxin expression vector which contains a chromosomal DNA sequence coding for human lymphotoxin and, a process for preparing human lymphotoxin, which comprises transforming cultured animal cell with a lymphotoxin expression vector which contains a chromosomal DNA sequence coding for human lymphotoxin, culturing the transformed cell culture to produce human lymphotoxin, and collecting the human lymphotoxin. According to the present invention, LT which is expected for application as the antitumor agent can be produced effectively in a large amount.

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