Lyophilized bioluminescent bacterial reagent for the...

Details Lyophilized bioluminescent bacterial reagent for the... Lyophilized bioluminescent bacterial reagent for the...

C - Chemistry, Metallurgy

12

Q

C12Q 1/02 (2006.01) C12N 1/04 (2006.01) C12N 9/02 (2006.01) C12Q 1/66 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2200702

A reagent, useful in the detection of environmental insults comprising bacterial cells containing a stress promoter operably linked to a lux gene complex has been prepared by lyophilizing the cells in a specified medium. The reagent may be used immediately upon rehydration where a positive test for the presence of an environmental insult is given by an increase in light production from the cells.

L'invention porte sur un réactif permettant de détecter des atteintes à l'environnement comprenant des cellules bactériennes contenant un promoteur de stress fonctionnellement liées à un complexe de gènes lux préparé par lyophilisation des cellules dans un milieu spécifique. Ledit réactif peut s'utiliser immédiatement après réhydratation. La positivité du test indiquant la présence d'une l'atteinte à l'environnement se manifeste par un accroissement de la production de lumière émise par les cellules.

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