Macromolecular environment control in specific receptor assays

Details Macromolecular environment control in specific receptor assays Macromolecular environment control in specific receptor assays

C - Chemistry, Metallurgy

12

Q

150/11, 150/3.3

C12Q 1/00 (2006.01) G01N 33/542 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1140033

ABSTRACT OF THE DISCLOSURE Method and compositions are provided for performing protein binding assays involving a homologous pair consisting of ligand and receptor for the ligand. The method employs a label conjugated to a member of said homologous pair and a uniformly dispersed discontinous phase of discrete particles in a continuous aqueous phase, where the discrete particles create microenvironments which allow for discrimination between the label associated with the particle--in a discontinuous phase--and the label in the continuous phase. Various conjugates and particles are provided which find use in the subject method.

325014

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