Magnetic device for isolation of cells and biomolecules in a...

Details Magnetic device for isolation of cells and biomolecules in a... Magnetic device for isolation of cells and biomolecules in a...

C - Chemistry, Metallurgy

12

Q

C12Q 1/00 (2006.01) C07H 21/00 (2006.01) C07K 1/02 (2006.01) C12N 1/00 (2006.01) C12N 5/00 (2006.01) C12N 7/00 (2006.01) C12N 15/10 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2557819

The present invention features a new and useful magnetic device and methods of its use for isolation, enrichment, and purification of cells, proteins, DNA, and other molecules. In general the device includes magnetic regions or obstacles to which magnetic particles can bind. The chemical groups, i.e., capture moieties, on the surface of the magnetic particles may then be used to bind particles, e.g., cells, or molecules of interest from complex samples, and the bound species may then be selectively released for downstream collection or further analysis.

L'invention concerne un nouveau dispositif magnétique utile et des procédés d'utilisation de celui-ci dans l'isolation, l'enrichissement et la purification de cellules, de protéines, d'ADN, et d'autres molécules. En général, ce dispositif comprend des zones magnétiques ou des obstacles auxquels les particules magnétiques peuvent se lier. Les groupes chimiques, notamment, des fractions de capture, sur la surface des particules magnétiques peuvent ensuite être utilisées pour lier des particules, notamment, des cellules, ou des molécules d'intérêt provenant d'échantillons complexes, et des espèces liées peuvent ensuite être libérées de façon sélective pour une collecte en aval ou une analyse plus approfondie.

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