Magnetic dna extraction kit for plants

Details Magnetic dna extraction kit for plants Magnetic dna extraction kit for plants

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01)

Patent

CA 2370656

A method and kit for the extraction of DNA from plants is provided, which quickly yields plant DNA with a high level of purity. The method isolates DNA (genomic, chloroplast, and/or mitochondrial DNA) using immobilized anionic groups, preferably on a chromatographic substrate or more preferably magnetic beads derivatized with anionic groups such as diethylaminoethyl (DEAE) via an anion-exchange interaction. The purified DNA is then eluted with ions (typically a salt solution). RNA can be removed by digestion with RNAse.

L'invention concerne un procédé et un kit pour l'extraction d'ADN de plantes, permettant de produire rapidement de l'ADN de plantes présentant un grand degré de pureté. Ledit procédé permet d'isoler de l'ADN (ADN génomique, de chloroplaste et/ou mitochondrien) au moyen de groupes anioniques immobilisés, de préférence sur un substrat chromatographique ou idéalement sur des billes magnétiques dérivées de groupes anioniques, tels que le diéthylaminoéthyle (DEAE), par une interaction à échange d'anions. L'ADN purifié est ensuite élué à l'aide d'ions (généralement une solution salée). L'ARN peut être supprimé par digestion à l'aide de Rnase.

Affiliated with

Also associated with

Rating

Magnetic dna extraction kit for plants does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Magnetic dna extraction kit for plants, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Magnetic dna extraction kit for plants will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-2015772