Magnetic isolation and purification of nucleic acids

Details Magnetic isolation and purification of nucleic acids Magnetic isolation and purification of nucleic acids

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) B01D 57/00 (2006.01) B03C 1/02 (2006.01) C07H 1/06 (2006.01) C12N 15/10 (2006.01) H01F 1/42 (2006.01)

Patent

CA 2438066

A method for the isolation and purification of nucleic acids such as DNA, RNA, and PNA from various sources using magnetizable cellulose or its derivatives. Adjusting the concentrations of the salt and polyalkylene glycol to the levels that result in binding of nucleic acids to the magnetizable cellulose or its derivatives. Separating the nucleic acids bound to the magnetizable cellulose particles or its derivatives and eluting the nucleic acids from the particles.

L'invention concerne un procédé destiné à l'isolement et à la purification d'acides nucléiques, tels que de l'ADN, de l'ARN et des PNA d'origines diverses, à l'aide de cellulose magnétisable ou de dérivés de cellulose. Ce procédé consiste à ajuster les concentrations du sel et du polyalkylène glycol aux niveaux conduisant à la fixation des acides nucléiques sur la cellulose magnétisable ou ses dérivés. Ce procédé consiste ensuite à séparer les acides nucléiques fixés sur les particules de cellulose magnétisable ou ses dérivés et à éluer les acides nucléiques retenus sur les particules.

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