Magnetic nanoparticles having biochemical activity, method...

A - Human Necessities – 61 – K

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A61K 47/48 (2006.01)

Patent

CA 2384429

The invention relates to magnetic nanoparticles, to the production thereof and to their use. The aim of the invention is to prepare nanoparticles which, also in the intracellular area of cells, can specifically bond to intracellular biomacromolecules so that a separation is made possible by the action of an external magnetic field. This is achieved by using magnetic nanoparticles which have a biochemical activity and which are comprised of a magnetic nuclear particle and of a shell layer that is fixed to the nuclear particle. The nanoparticles contain a compound of general formula M - S - L - Z (I), whereby the binding sites between S and L and L and Z have covalently bound functional groups. M represents the magnetic nuclear particle, S represents a biocompatible substrate fixed to M, L represents a linker grouping, and Z represents a grouping, which is comprised of nucleic acids, peptides or proteins or of their derivatives, and which has at least one structure that is specifically capable of binding with a binding domain of an intracellular biomacromolecule.

L'invention concerne des nanoparticules magnétiques à effet biochimique, leur procédé de production et leur utilisation. L'invention vise à mettre à disposition des nanoparticules qui, même à l'intérieur de cellules, peuvent se lier de manière spécifique à des biomolécules intracellulaires, de façon à permettre une séparation sous l'effet d'un champ magnétique extérieur. A cet effet, on fait appel à des nanoparticules magnétiques à effet biochimique, constituées d'un noyau magnétique et d'une enveloppe fixée sur ce dernier. Ces nanoparticules contiennent un composé de formule générale M - S - L - Z (I), les sites de liaison entre S et L et entre L et Z présentant des groupes fonctionnels liés par covalence. Dans la formule, M représente le noyau magnétique, S un substrat biocompatible fixé sur M, L un groupement de séquences de liaison, et Z un groupe constitué d'acides nucléiques, de peptides ou de protéines ou bien de leurs dérivés, ledit groupement présentant au moins une structure pouvant se lier de manière spécifique à un domaine de liaison d'une biomolécule intracellulaire.

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