Making influenza virus vaccines without using eggs

A - Human Necessities – 61 – K

Patent

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Details

A61K 39/145 (2006.01) C07K 14/10 (2006.01)

Patent

CA 2663196

Currently, the steps performed prior to release of influenza strains to vaccine manufacturers involve passaging influenza virus through eggs. The invention aims to provide procedures useful in manufacturing influenza vaccines, in which the use of eggs is reduced, and preferably is avoided altogether. For instance, rather than use chicken eggs for influenza vaccine isolation, MDCK cells (Madin Darby canine kidney cells) may be used e.g. growing in suspension, growing in a serum-free medium, growing in a protein-free medium, being non-tumorigenic, grown in the absence of an overlay medium, etc.

A l'heure actuelle, les étapes réalisées avant la livraison de souches grippales aux fabricants de vaccins comprennent le passage du virus grippal dans des AEufs. La présente invention concerne des procédures utilisables dans la fabrication de vaccins antigrippaux dans lesquelles l'utilisation d'AEufs est réduite ou est de préférence totalement évitée. Par exemple, au lieu d'utiliser des AEufs de poule pour l'isolement du vaccin antigrippal, on peut utiliser des cellules rénales canines Madin-Darby (cellules MDCK : Madin Darby Canine Kidney) par exemple cultivées en suspension, cultivées dans un milieu sans sérum, cultivées dans un milieu sans protéine, non tumorigènes, cultivées en l'absence d'un milieu de recouvrement, etc.

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