Malaria recombinant poxvirus vaccine

C - Chemistry – Metallurgy – 12 – N

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C12N 7/01 (2006.01) A61K 39/015 (2006.01) C07K 14/445 (2006.01) C12N 15/30 (2006.01) C12N 15/86 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2168124

What is described is a recombinant poxvirus, such as vaccinia or canarypox virus, containing foreign DNA from Plasmodium such as coding for at least one of CSP, PfSSP2, LSA-I, LSA-1-repeatless, MSA-1, SERA, AMA-1, Pfs25, MSA-1 N-terminal p83 and MSA-1 C-terminal gp42. What is also described is a vaccine containig the recombinant poxvirus for inducing an immunological response in a host animal inoculated with the vaccine. Preferred recombinants have attenuated virulence. In certain embodiments the vaccinia has deleted or disrupted the thymidine kinase gene, the hemorrhagic region, the A type inclusion body region, the host range gene region and, the large subunit, ribonucleotide reductase; and, contains coding sequences for CSP, PfSSP2, LSA-1-reoeatless, MSA-1, SERA, AMA-1 and Pfs25. That embodiment is termed NYVAC-Pf7 and is a multicomponent, multistage vaccine since it codes for and expresses sporozoite proteins, liver stage proteios, blood stage proteins and, sexual stage proteins.

Un poxvirus recombiné est décrit, tel que le virus de la vaccine ou du canarypox, contenant de l'ADN étranger de Plasmodium, tel que celui qui code pour l'un au moins de CSP, PfSSP2, LSA-1, LSA-1- sans répétition, MSA-1, SERA, AMA-1, Pfs25, MSA-1 N-terminal p83 et MSA-1 C-terminal gp42. Est également décrit un vaccin contenant le poxvirus recombiné en vue d'induire une réponse immunologique chez un animal hôte auquel le vaccin est inoculé. Les produits recombinés préférés ont une virulence atténuée. Dans certains modes de réalisation du virus de la vaccine, le gène thymidine kinase, la région hémorragique, la région à corps d'inclusion de type A, la région du gène du spectre d'hôte et la grosse sous-unité ribonucléotide-réductase sont supprimés ou rompus; et il contient des séquences de codage pour CSP, PfSSP2, LSA-1-sans répétition, MSA-1, SERA, AMA-1 et Pfs25. Ce mode de réalisation est appelé NYVAC-Pf7 et c'est un vaccin à plusieurs composants et plusieurs stades car il code et exprime les protéines des sporozoïtes, les protéines du stade hépatique, les protéines du stade sanguin, les protéines du stade sexuel.

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