Malathion carboxylesterase

Details Malathion carboxylesterase Malathion carboxylesterase

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) C12S 99/00 (2010.01) B09C 1/10 (2006.01) C02F 1/58 (2006.01) C02F 3/00 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/18 (2006.01)

Patent

CA 2236793

The present invention provides an isolated DNA molecule encoding Malathion Carboxylesterase capable of hydrolysing at least one organophosphate selected from the group consisting of carboxylester organophosphates and dimethyloxon organophosphates. The DNA molecule comprises a nucleotide sequence having at least 60 %, preferably at least 80 % and more preferably at least 95 % homology with Lc.alpha.E7, in which the protein encoded by the DNA molecule differs from E3 at least in the substitution of Trip at position 251 with an amino acid selected from the group consisting of Leu, Ser, Ala, Ile, Val, Thr, Cys, Met and Gly. The preferred substituents are Leu and Ser.

La présente invention concerne une molécule d'ADN isolée codant la carboxylestérase agissant sur le malathion et capable d'hydrolyser l'un au moins des organophosphates appartenant au groupe des carboxylester organophosphates et dyméthyloxone organophosphates. La molécule d'ADN comprend une séquence nucléotide présentant une homologie au moins à 60 %, de préférence au moins à 80 % et encore mieux au moins à 95 % avec Lc alpha E7, séquence dans laquelle la protéine codée par la molécule d'ADN diffère de E3 au moins dans la substitution du Trip en position 251 par un acide aminé choisi dans le groupe des Leu, Ser, Ala, Ile, Val, Thr, Cys, Met et Gly, les substituants préférés étant Leu et Ser.

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