Male ferility restoration as a selectable marker in plant...

A - Human Necessities – 01 – H

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A01H 1/02 (2006.01) C12N 15/31 (2006.01) C12N 15/55 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2496537

The present invention provides methods of identifying transformed plants using the restoration of male fertility as a selectable marker. Preferably, the fertility restorer genes are used to restore fertility in Brassica napus. The present invention also includes vectors comprising male sterility genes flanked by recombinase sites, vectors comprising a fertility restorer gene and a nucleotide sequence of interest flanked by recombinase sites, and methods of using such vectors to produce transgenic plants, using restoration of male fertility as the selection for transformation events.

Cette invention se rapporte à des procédés permettant d'identifier des plantes transformées grâce à la restauration de la fécondité mâle utilisée comme marqueur sélectionnable. Les gènes de restauration de la fécondité sont de préférence utilisés pour restaurer la fécondité dans l'espèce Brassica napus. Cette invention concerne également des vecteurs comprenant des gènes de stérilité mâles encadrés par des sites de recombinase, des vecteurs comprenant un gène de restauration de la fécondité et une séquence nucléotidique d'intérêt encadrée par des sites de recombinase, ainsi que des procédés utilisant ces vecteurs pour produire des plantes transgéniques, grâce à la restauration de la fécondité mâle utilisée comme sélection pour des événements de transformation.

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