Male infertility y-deletion detection battery

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2271948

The present disclosure discloses a method for probing the integrity of a Y chromosome utilizing multiplex PCR reactions which amplify specific regions of the human Y chromosome which have been linked to normal fertility in human males. The method is capable of detecting deletion mutations within the Y chromosome which are predictive of human male infertility. A kit containing reagents needed to practice the method is also disclosed.

La présente invention concerne une méthode de sondage de l'intégrité d'un chromosome Y par amplification en chaîne par polymérase multiplex pour amplifier des régions spécifiques du chromosome Y humain liées à la fertilité normale chez des humains de sexe masculin. La méthode de l'invention permet de déterminer des mutations de délétion dans le chromosome Y, mutations qui sont prédictives de l'infertilité chez des humains de sexe masculin. L'invention concerne également une trousse contenant des réactifs nécessaires pour mettre en oeuvre la méthode.

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