Malic acid production in recombinant yeast

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/00 (2006.01) C12N 1/16 (2006.01) C12P 7/46 (2006.01)

Patent

CA 2630333

We disclose a recombinant yeast, wherein the yeast is pyruvate decarboxylase enzyme (PDC) activity negative (PDC-negative) and is functionally transformed with a coding region encoding a pyruvate carboxylase enzyme (PYC) wherein the PYC is active in the cytosol, a coding region encoding a malate dehydrogenase enzyme (MDH) wherein the MDH is active in the cytosol and is not inactivated in the presence of glucose, and a coding region encoding a malic acid transporter protein (MAE). We also disclose a method of producing malic acid by culturing such a yeast in a medium comprising a carbon source and a carbon dioxide source and isolating malic acid from the medium.

La présente invention concerne une levure recombinante à l'activité pyruvate decarboxylase (PDC) négative. Cette levure fonctionnellement transformée présente ainsi une région codante codant une pyruvate carboxylase (PYC) et dans laquelle la PYC est active dans le cytosol, une région codante codant une malate déshydrogénase (MDH) et dans laquelle la MDH est active dans le cytosol sans être inactivée en présence de glucose, et une région codante codant un transporteur d'acide malique (MAE). L'invention concerne également un procédé de production d'acide malique, par culture d'une telle levure dans un milieu comrpenant une source de carbone et une source de dioxyde de carbone, puis par séparation de l'acide malique du milieu de culture.

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