Maltogenic alpha-amylase variants

Details Maltogenic alpha-amylase variants Maltogenic alpha-amylase variants

C - Chemistry, Metallurgy

12

N

C12N 9/28 (2006.01) C12N 9/26 (2006.01) C12N 15/00 (2006.01) C12N 15/56 (2006.01)

Patent

CA 2321595

The inventors have modified the amino acid sequence of a maltogenic alpha- amylase to obtain variants with improved properties, based on the three- dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties., e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.

Selon cette invention, on a modifié la séquence d'acides aminés d'une alpha-amylase maltogène pour obtenir des variantes aux propriétés améliorées, sur la base de la structure tridimensionnelle de Novamyl alpha-amylase maltogène. Les variantes ont modifié les propriétés physico-chimiques pour donner, par exemple, un pH optimum modifié, une meilleure thermostabilité, une activité spécifique accrue, un schéma de clivage modifié ou une capacité accrue à réduire la rétrogradation de l'amidon ou le rassissement du pain.

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