Maltogenic alpha-amylase variants

Details Maltogenic alpha-amylase variants Maltogenic alpha-amylase variants

C - Chemistry, Metallurgy

12

N

C12N 9/28 (2006.01) A21D 8/04 (2006.01) C12N 15/56 (2006.01) C12P 7/06 (2006.01) C12P 19/12 (2006.01)

Patent

CA 2575875

The inventors realized that in some applications the control of the maltose-to- glucose ratio is of great importance. Particularly for ethanol production from granular starch by fermen-tation, it may be an advantage to form a larger amount of glucose which is more readily fermentable than maltose. Particularly for production of maltose syrups glucose is an undesired product, and hence it of interest to increase the maltose-to-glucose ratio. They then developed a method of constructing such variants of based on the three-dimensional structure of a parent maltogenic alpha-amylase.

Selon l'invention, dans certaines applications, la régulation du rapport du maltose au glucose est d'une grande importance. En particulier, pour la production d'éthanol par fermentation à partir d'amidon perlé, il peut être avantageux de produire une plus grande quantité de glucose qui fermente plus facilement que le maltose. En particulier, pour la production de sirops de maltose, le glucose est un produit inapproprié, et il importe donc d'augmenter le rapport du maltose au glucose. Un procédé a ainsi été mis au point pour construire des tels variants fondés sur la structure tridimensionnelle d'une alpha-amylase maltogène parente.

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