Mammalian artificial chromosomes

Details Mammalian artificial chromosomes Mammalian artificial chromosomes

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) C12N 15/68 (2006.01) C12N 15/81 (2006.01) C12N 15/90 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2263823

A mammalian chromosome is produced according to the method comprising the steps of: introducing a DNA construct comprising a mammalian telomere and a centromere into a mammalian cell, wherein the centromere has a DNA sequence containing some copies of CENP-B box sequence consisting of. 5'-NTTCGNNNNANNCGGGN-3', wherein N is any one of A, T, C and G.

Un chromosome de mammifère est produit selon le procédé consistant à introduire un ADN de recombinaison comportant un télomère de mammifère et un centromère dans une cellule de mammifère, ledit centromère possédant une séquence nucléotidique contenant quelques copies de la boîte CENP-B présentant la constitution suivante: 5'-NTTCGNNNNANNCGGGN-3', où N peut représenter A, T, C ou G.

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