C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/85 (2006.01) C07K 7/08 (2006.01) C12N 15/62 (2006.01) C12P 21/02 (2006.01) C07K 16/00 (2006.01)
Patent
CA 2687921
The present invention relates to a mammalian cell based expression and secretion system and the expression and secretion of recombinant proteins by using secretory signal peptides. The present invention also relates to an expression cassette useful for the secretion of a heterologous gene from a mammalian cell, in particular a CHO cell. The present invention is also directed to a method of secreting a heterologous protein from mammalian cells such as CHO cells.
La présente invention porte sur un système d'expression et de sécrétion à base de cellule de mammifère ainsi que sur l'expression et la sécrétion de protéines recombinantes par peptides signaux sécrétoires. La présente invention porte également sur une casette d'expression utile pour la sécrétion d'un gène hétérologue provenant d'une cellule de mammifère, en particulier une cellule CHO. La présente invention porte également sur un procédé de sécrétion d'une protéine hétérologue provenant de cellules de mammifère telles que des cellules CHO.
Rance James
Young Robert
G. Ronald Bell & Associates
Lonza Biologics Plc
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