Mammalian expression vectors

Details Mammalian expression vectors Mammalian expression vectors

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) C07K 14/54 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2076975

2076975 9113160 PCTABS00007 This invention provides novel recombinant plasmids which can be used to clone heterologous genes in E. coli and to clone and express such genes in mammalian cells. These plasmids contain a number of unique restriction sites that can be used to facilitate the deletion or replacement of genetic elements and the insertion of heterologous genes in the plasmids. The nature and placement of the unique sites is such that regeneration of the sites following endonuclease cleavage can readily be achieved with a minimum of manipulative effort. Host cells containing the recombinant plasmids are also provided by this invention.

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