Mammalian gamete and embryo culture media supplement and...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/073 (2010.01)

Patent

CA 2410931

The present invention provides a supplement and a culture media useful for culturing mammalian gametes and embryonic tissue. The culture media comprises at least one of recombinant human albumin, fermented hyaluronan, and citrate. Because the constituents are produced from non-conventional sources, the culture medium is free from contaminants such as viruses, prions and endotoxins. Additionally, because the medium is completely defined, the medium is not subject to variations which can impair the development of mammalian cells and prevent meaningful comparisons of empirical studies.

La présente invention concerne un supplément et un milieu de culture utilisé dans la culture de gamètes et de tissus embryonnaires mammaliens. Le milieu de culture renferme au moins une albumine humaine recombinante, de l'hyaluronan fermenté, et du citrate. Etant donné que les constituants sont produits à partir de sources non traditionnelles, le milieu de culture est exempt de contaminants, tels que des virus, des prions et des endotoxines. En outre, comme le milieu est entièrement défini, il n'est pas soumis aux variations qui peuvent nuire au développement des cellules mammaliennes et empêcher des comparaisons pertinentes d'études empiriques.

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