Mammalian neuralized family transcriptional regulators and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) A61K 31/7088 (2006.01) A61K 38/17 (2006.01) A61K 48/00 (2006.01) C07K 14/47 (2006.01) C07K 14/71 (2006.01) C07K 14/82 (2006.01) C12N 15/63 (2006.01) C12N 15/64 (2006.01) C12N 15/85 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2455413

The disclosure relates to isolated polynucleotides and purified polypeptides of the Neu family of proteins, which have been shown to demonstrate transcriptional regulatory activity. For example, the purified polynucleotide can encode a Neu polypeptide, wherein the Neu polypeptide comprises at least one neuralized homology repeat domain and a C3HC4 RING-zinc finger domain is disclosed. A purified Neu polypeptide, wherein the Neu polypeptide comprises at least one neuralized homology repeat domain and a C3HC4 RING-zinc finger domain is disclosed. Antibodies capable of specifically binding to the disclosed Neu polypeptides are disclosed. Vectors expressing the disclosed Neu protein coding regions and host cells containing the vectors are disclosed. Methods of making the Neu proteins disclosed are also provided, as are method of identifying binding partners that interact with a Neu protein family member.

La présente invention concerne des polynucléotides isolés et des polypeptides purifiés de la famille protéique Neu, ayant une activité régulatrice transcriptionnelle. Par exemple, le polynucléotide purifié peut coder pour un polypeptide Neu, le polypeptide Neu comprenant au moins un domaine de répétition présentant une homologie avec Neu et un domaine en doigt de zinc C3HC4 RING. Un polypeptide Neu purifié comprenant au moins un domaine de répétition présentant une homologie avec Neu et un domaine en doigt de zinc C3HC4 RING. Des anticorps pouvant se fixer spécifiquement sur les polypeptides Neu susmentionnés. Des vecteurs exprimant les régions de codage des protéines Neu et des cellules hôtes contenant les vecteurs. La présente invention concerne également des procédés permettant d'élaborer les protéines Neu, ainsi que des procédés permettant d'identifier les partenaires de liaison qui interagissent avec un membre de la famille protéique Neu.

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