Mammalian-type glycosylation in plants by expression of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/00 (2006.01)

Patent

CA 2684370

The present invention relates to non-mammalian .beta.-l,4-galactosyltransferases that can be used in their wild-type or in modified forms. The invention further relates to transformed plants and plant cells expressing non-mammalian .beta.-l,4-galactosyltransferases and methods to produce glycoproteins with altered and preferably mammalian-type glycosylation. The invention additionally provides nucleic acid molecules and expression vectors of non-mammalian .beta.-l,4-galactosyltransferases.

La présente invention porte sur des â-l,4-galactosyltransférases non mammifères pouvant être utilisées dans leur type sauvage ou dans des formes modifiées. L'invention porte en outre sur des plantes transformées et des cellules de plantes exprimant des â-l,4-galactosyltransférases non mammifères, et sur des procédés de fabrication de glycoprotéines avec une glycosylation modifiée et, de préférence, de type mammifère. L'invention porte en outre sur des molécules d'acide nucléique et des vecteurs d'expression de â-l,4-galactosyltransférases non mammifères.

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