Manganese lipoxygenase

Details Manganese lipoxygenase Manganese lipoxygenase

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) A21D 10/00 (2006.01) A21D 13/00 (2006.01) C07H 21/00 (2006.01) C11D 1/22 (2006.01) C11D 3/386 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 9/04 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12P 7/04 (2006.01) C12P 7/40 (2006.01) C12P 7/62 (2006.01) C12P 21/02 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2419577

The invention provides sequence information of a microbial protein having lipoxy-genase activity and a method of producing the protein by recombinant DNA technology. More specifically, the inventors have isolated a gene encoding a lipoxygenase from Gaeu-mannomyces graminis, cloned it into an E. coli strain and sequenced it. A comparison shows less than 25 % identity to known lipoxygenase sequences, the closest being human 15S li-poxygenase. The inventors have expressed the lipoxygenase recombinantly and found that the recombinant lipoxygenase is glycosylated.

L'invention concerne des informations relatives à la séquence d'une protéine microbienne exerçant une activité de lipoxygénase et concerne un procédé de production de la protéine par génie génétique. Plus précisément, les inventeurs ont isolé un gène codant une lipoxygénase issue de Gaeumannomyces graminis, puis l'ont cloné dans une souche E.coli et l'ont séquencé. Une comparaison fait apparaître moins de 25 % d'identité avec les séquences de lipoxygénase connues, la plus proche étant la lipoxygénase humaine 15S. Les inventeurs ont exprimé la lipoxygénase par recombinaison et ont découvert que la lipoxygénase recombinée est glycosylée.

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