Manipulating isoprenoid expression

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) A01H 5/00 (2006.01) C12N 1/21 (2006.01) C12N 9/10 (2006.01) C12N 15/82 (2006.01) C12Q 1/48 (2006.01)

Patent

CA 2360334

There is disclosed a method of manipulating isoprenoid expression in a cell or organism having a mevalonate independent isopentyl diphosphate synthesising pathway, which method comprises altering the activity of the enzyme 1-deoxy-D- xylulose-5-phosphate synthase (DXPS), or a functional equivalent, derivative or bioprecursor thereof. Also disclosed is a transgenic cell, tissue or organism having a mevalonate independent IPP biosynthetic pathway which cell, tissue or organism comprises at least one transgene capable of expressing DXPS or a functional equivalent, derivative or bioprecursor thereof.

L'invention concerne une technique permettant de manipuler l'expression d'un isoprénoïde dans une cellule ou dans un organisme possédant une voie de synthèse du diphosphate isopentyle indépendante du mévalonate. Cette technique consiste à altérer l'activité de l'enzyme 1-déoxy-D-xylulose-5-phosphate synthase (DXPS), ou d'un équivalent fonctionnel, d'un dérivé ou d'un bioprécurseur de celui-ci. L'invention concerne également une cellule transgénique, un tissu ou un organisme comprenant une voie de biosynthèse IPP indépendante du mévalonate, lesquels cellule, tissu ou organisme comprennent au moins un transgène capable d'exprimer DXPS ou un équivalent fonctionnel, un dérivé ou un bioprécurseur de celui-ci.

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