C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) B01J 20/22 (2006.01) B01J 20/32 (2006.01) B01L 3/00 (2006.01) B01L 7/00 (2006.01) C07H 21/00 (2006.01)
Patent
CA 2126748
ABSTRACT OF THE DISCLOSURE A method of effecting a manipulation of nucleic acid sequence comprises (a) providing a solid support system having bonded thereto a single stranded oligonucleotide complementary to a specific sequence on a target nucleic acid longer than said oligonucleotide, (b) adding a source of single stranded target nucleic acid to the solid support system, (c) hybridising the target nucleic to the oligonucleotide, and (d) effecting the manipulation of the hybridised target nucleic acid. The manipulation may for example be a copying or amplification of the target nucleic acid sequence. In a preferred embodiment, the support is provided in a flow-through vessel which facilitates washing of the support to remove impurities and leave a "clean" sample of target nucleic acid on which the manipulation may be carried out. The support preferably has a siloxane matrix to which the oligonucleotide is bound. This provides a stable linkage between the oligonucleotide and the support. The method is particularly useful for analysis of medical samples.
Marks & Clerk
Tepnel Medical Limited
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